随着年龄的增长,人体皮肤因自身生理调节和外界因素的作用而逐渐出现雀斑、日晒斑、黄褐斑等色斑,色斑的形成与皮肤中黑色素的沉着有关,祛斑功效实质上是对黑色素的清除作用。通过班斑马鱼技术能简洁而有效的评价化妆品的祛斑功效。
【评价原理】
斑马鱼在发育初期全身透明,胚胎发育至 24 h时黑色素开始从视网膜上皮生长,48h逐渐形成稳定并可见的黑色素色斑。将受试物于斑马鱼发育48h时加入鱼水中,72h时观察黑色素的降低程度,从而评价祛斑功效
【实验方案】
我们挑选2dpf的斑马鱼分成若干组,含正常对照组和供试品组。正常对照组不做处理,供试品组使用受试化妆品,孵育24hpf后,对斑马鱼头部进行拍照,利用高级图像处理软件分析斑马鱼头部的黑色素信号强度,从而评价其祛斑功效。
【结果展示】(展示图片仅供参考,实际实验组别依据合同而定)
图1. 斑马鱼头部黑色素表型图(蓝色为分析区域)
阳性对照组和化妆品组与正常对照组对比分析,得出祛斑功效作用。
【评价结论】
1.经过各组斑马鱼对比实验,阳性对照组和化妆品组与正常对照组对比,斑马鱼头部只有少量的黑色素。
2.本实验证实了该化妆品具有祛斑作用。
【参考文献】
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